Human activities have severe impacts on the biological carbon and nitrogen cycles. The most important consequences of these are global warming and water pollution. Wastewater treatment technology, in particular nitrogen removal systems, improved considerably in the last decade. The application of anaerobic ammonium oxidizing (anammox) bacteria in oxygen-limited granules has the potential to turn wastewater treatment plants into energy-efficient systems with minimal greenhouse gas emissions of carbon dioxide, nitrous oxide and methane. Recently, microorganisms that couple the anaerobic oxidation of methane to denitrification were discovered. An innovative integration of these microorganisms into partial nitritation-anammox systems for wastewater treatment offers an elegant and efficient solution to combat greenhouse gas emissions from wastewater treatment plants. The aim of the GREENT project is to determine nitrous oxide emissions from partial nitritation-anammox bioreactors and the parameters that govern these emissions, and to investigate the responsible pathways in molecular detail. Furthermore, I will explore the feasibility of an innovative bioreactor, which will remove ammonium and methane simultaneously through anammox and anaerobic methane-oxidizing microorganisms. This approach will turn the otherwise harmful methane into a resource for efficient nitrogen removal. These fundamental insights and technological advances will be achieved by a complimentary array of state-of-the-art methodology such as continuous culturing, stable isotope tracing and a combination of -omics methods together with physiological experiments and detailed measurements at full-scale installations. The results of this proposal will be of paramount importance for the wastewater treatment sector, but also indispensable for mathematical modelling, life cycle assessment and understanding the ecophysiology of the involved microorganisms.
- Abteilungen
- Abteilung Biogeochemie
- Forschungsgruppe Mikrobielle Physiologie
- ERC Project – GREENT
ERC Project – GREENT
Gruppenleiter
Forschungsgruppe Mikrobielle Physiologie
MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany
Raum: |
3126 |
Telefon: |
Scientists
Forschungsgruppe Mikrobielle Physiologie
MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany
Raum: |
3137 |
Telefon: |
Press Releases
- When methane-eating microbes eat ammonia instead
- link to related paper: Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs
- Discovery of an unusual protein
- link to related paper: Discovery of a functional, contracted heme-binding motif within a multiheme cytochrome
- Fuel cells in Bacteria
- link to related paper: A 192-heme electron transfer network in the hydrazine dehydrogenase complex
- Microbes can grow on Nitric Oxide
- link to related paper: Nitric oxide-dependent anaerobic ammonium oxidation
- Microbe hunters discover long-sought-after iron-munching microbe
- link to related paper: Archaea catalyze iron-dependent anaerobic oxidation of methane
Popularized Publications
Publications
2021 | Learning from microorganisms: using new insights in microbial physiology for sustainable nitrogen management P. Garrido-Amador, M. Kniaziuk, B. Vekeman B. Kartal Current Opinion in Biotechnology, vol. 67, pp. 42 – 48. link to paper |
2020 | Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs W. Versantvoort, A. Pol, M.S.M. Jetten, L. van Niftrik, J. Reimann, B. Kartal, H. J. M. op den Camp Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 39, pp. 24459 – 24463. link to paper |
Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant K. Stultiens, M. A. H. J. van Kessel, J. Frank, P. Fischer, C. Pelzer, T. A. van Alen, B. Kartal, H. J. M. op den Camp, M.S. M. Jetten Applied Microbiology and Biotechnology, vol. 104, no. 16, pp. 7201 – 7212. link to paper |
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2019 | Discovery of a functional, contracted heme-binding motif within a multiheme cytochrome C. Ferousi, S. Lindhoud, F. Baymann, E. R. Hester, J. Reimann, B. Kartal Journal of Biological Chemistry, vol. 294, no. 45, pp. 16953-16965 link to paper |
A 192-heme electron transfer network in the hydrazine dehydrogenase complex M. Akram, A. Dietl, U. Mersdorf, S. Prinz, W. Maalcke, J. Keltjens, C. Ferousi, De Almeida, N.M., J. Reimann, B. Kartal, M.S.M. Jetten, K. Parey, T.R.M. Barends Science Advances, vol. 5, no. 4 link to paper |
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A 60-heme reductase complex from an anammox bacterium shows an extended electron transfer pathway A. Dietl, W.J. Maalcke, C. Ferousi, M.S.M. Jetten, B. Kartal, T.R.M. Barends Acta Crystallographica Section D: Structural Biology, vol. 75, no. 3, pp. 333–341. link to paper |
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Arcobacter peruensis sp.nov., a chemolithoheterotroph isolated from sulfide and organic rich coastal waters off Peru C.M. Callbeck, C. Pelzer, G. Lavik, T.G. Ferdelmann, J.S. Graf, B. Vekeman, H. Schunck, S. Littmann, B.M. Fuchs, P.F. Hach, T. Kalvelage, R.A. Schmitz, M.M.M .Kuypers Applied and Environmental Microbiology, vol. 85, issue. 24 link to paper |
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Methyloparacoccus B. Vekeman Bergey's Manual of Systematics of Archaea and Bacteria, link to paper |
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A nitric oxide-binding heterodimeric cytochrome c complex from the anammox bacterium Kuenenia stuttgartiensis binds to hydrazine synthase M. Akram, J. Reimann, A. Dietl, A. Menzel, W. Versantvoort, B. Kartal, M.S.M. Jetten, T.R.M. Barends Journal of Biological Chemistry, vol. 294, no. 45, pp. 16712–16728. link to paper |
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Complexome analysis of the nitrite-dependent methanotroph Methylomirabilis lanthanidiphila W. Versantvoort, S. Guerrero-Castillo, H.J.C.T. Wessels, van Niftrik, L., M.S.M. Jetten, U. Brandt, J. Reimann, B. Kartal Biochimica et Biophysica Acta - Bioenergetics, vol. 1860, no. 9, pp. 734–744. link to paper |
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Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor K. Stultiens, S.G. Cruz, van Kessel, M.A.H.J., M.S.M. Jetten, B. Kartal, Op den Camp, H.J.M. Applied Microbiology and Biotechnology, vol. 103, no. 16, pp. 6783–6795. link to paper |
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Interactions of anaerobic ammonium oxidizers and sulfide-oxidizing bacteria in a substrate-limited model system mimicking the marine environment L. Russ, van Alen, T.A., M.S.M. Jetten, Op den Camp, H.J.M., B. Kartal FEMS microbiology ecology, vol. 95, no. 9. link to paper |
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Key physiology of a nitrite-dependent methane-oxidizing enrichment culture S. Guerrero-Cruz, K. Stultiens, van Kessel, M.A.H.J., W. Versantvoort, M.S. Jetten, den Camp, H.J.M.O., B. Kartal Applied and Environmental Microbiology, vol. 85, no. 8. link to paper |
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Nitric oxide-dependent anaerobic ammonium oxidation Z. Hu, H.J.C.T. Wessels, van Alen, T., M.S.M. Jetten, B. Kartal Nature Communications, vol. 10, no. 1 link to paper |
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2018 | Community composition and ultrastructure of a nitrate-dependent anaerobic methane-oxidizing enrichment culture L. Gambelli, S. Guerrero-Cruz, R.J. Mesman, G. Cremers, M.S.M. Jetten, Op den Camp, H.J.M., B. Kartal, C. Lueke, van Niftrik, L. Applied and Environmental Microbiology, vol. 84, no. 3. link to paper |
Comparative genomics of Candidatus Methylomirabilis species and description of Ca. Methylomirabilis lanthanidiphila W. Versantvoort, S. Guerrero-Cruz, D.R. Speth, J. Frank, L. Gambelli, G. Cremers, van Alen, T., M.S.M. Jetten, B. Kartal, Op den Camp, H.J.M., J. Reimann Frontiers in Microbiology, vol. 9, link to paper |
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Current perspectives on the application of N-damo and anammox in wastewater treatment van Kessel, M.A., K. Stultiens, M.F. Slegers, Guerrero Cruz, S., M.S. Jetten, B. Kartal, Op den Camp, H.J. Current Opinion in Biotechnology, vol. 50, pp. 222–227. link to paper |
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The microbial nitrogen-cycling network M.M.M. Kuypers, H.K. Marchant, B. Kartal Nature Reviews Microbiology, vol. 16, no. 5, pp. 263–276. free to view link, link to paper |
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2017 | Iron assimilation and utilization in anaerobic ammonium oxidizing bacteria C. Ferousi, S. Lindhoud, F. Baymann, B. Kartal, M.S. Jetten, J. Reimann Current Opinion in Chemical Biology, vol. 37, pp. 129–136. link to paper |
2016 | Anammox biochemistry: a tale of heme c proteins B. Kartal, J.T. Keltjens Trends in Biochemical Sciences, vol. 41, no. 12, pp. 998–1011. link to paper |
Archaea catalyze iron-dependent anaerobic oxidation of methane K.F. Ettwig, B. Zhu, D. Speth, J.T. Keltjens, M.S.M. Jetten, B. Kartal Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 45, pp. 12792–12796. link to paper |
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Characterization of anammox hydrazine dehydrogenase, a key N2-producing enzyme in the global nitrogen cycle W.J. Maalcke, J. Reimann, De Vries, S., J.N. Butt, A. Dietl, N. Kip, U. Mersdorf, T.R.M. Barends, M.S.M. Jetten, J.T. Keltjens, B. Kartal Journal of Biological Chemistry, vol. 291, no. 33, pp. 17077–17092. link to paper |
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Membrane-bound electron transport systems of an anammox bacterium: A complexome analysis de Almeida, N.M., H.J.C.T. Wessels, de Graaf, R.M., C. Ferousi, M.S.M. Jetten, J.T. Keltjens, B. Kartal Biochimica et Biophysica Acta - Bioenergetics, vol. 1857, no. 10, pp. 1694–1704. link to paper |