- Departments
- Department of Molecular Ecology
- Molecular Ecology
- Superresolution Microscopy
Superresolution Microscopy
Superresolution microscopy allows us to visualize microstructures below the diffraction limit.
The techniques Superresolution Structured Illumination (SR-SIM) and Photoactivated Localization Microscopy (PALM) are combined with our confocal laser scanning microscope. more ...
Our latest superresolution instrument is based on the Stimulated Emission Depletion (STED)[1] technique.
The Abberior Instruments easy3D STED can provide a lateral resolution below 25 nm and a 3D resolution of up to 60 nm.
The instrument includes the methodes pulsed-STED[2], gated-STED[3],[4], and RESCue STED[5]. It is the first STED microscope with MINFIELD[6] technique on the commercial market.
Technical features
Excitation laser | STED laser | Detection* | |||
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450/50 nm |
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(pulsed, 1 W) |
509/22 nm |
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525/50 nm or |
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518 nm (pulsed, 300 µW) |
545/24 nm |
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(pulsed, 3 W) |
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615/20 nm | |||||
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*single-photon-counting avalanche photodiode (apd module)
Reservation
Location
Room 2242, Phone 931
Responsible
References
1. | Hell, S.W., J. Wichmann. (1994). Breaking the diffraction resolution limit by stimulated emission: Stimulated-emission-depletion fluorescence microscopy. Optics Letters. 19: 780–82. (doi:10.1364/OL.19.000780). |
2. | Dyba, M., S. W. Hell. (2003). Photostability of a Fluorescent Marker Under Pulsed Excited-State Depletion through Stimulated Emission. Applied Optics. 42:5123–29. (doi:10.1364/AO.42.005123). |
3. | Vicidomini, G., G. Moneron, K.Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, and S.W. Hell. (2011). Sharper low-power STED nanoscopy by time gating. Nat. Meth. 8:571–3. (doi:10.1038/nmeth.1624). |
4. | Moffitt, J.R., C. Osseforth, and J. Michaelis. (2011). Time-gating improves the spatial resolution of STED microscopy. Opt. Express. 19:4242–54. (doi:10.1364/OE.19.004242). |
5. | Staudt, T., A. Engler, E. Rittweger, B. Harke, J. Engelhardt, S.W. Hell, (2011). Far-field optical nanoscopy with reduced number of state transition cycles. Opt. Express. 19:5644–57. (doi:10.1364/OE.19.005644). |
6. | Göttfert, F., T. Pleiner, J. Heine, V. Westphal, D. Görlich, S.J. Sahl, S.W. Hell. (2017). Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc. Natl. Acad. Sci. USA. 114:2125-30. (doi:10.1073/pnas.1621495114). |